Effect of Squid Powder (Loligo Sp.) On Antibody Titer and Bacteria Density in Blood of Tilapia (Oreochromis niloticus) infected by Aeromonas hydrophila.

Mohamad Fadjar; Ellana Sanoesi; Yoga Aris Mintiya; Lukmanul Hakim

doi:10.21534/ai.v21i1.170

Effect of Squid Powder (Loligo Sp.) On Antibody Titer and Bacteria Density in Blood of Tilapia (Oreochromis niloticus) infected by Aeromonas hydrophila.

Mohamad Fadjar, Ellana Sanoesi, Yoga Aris Mintiya, Lukmanul Hakim

Abstract

Aeromonas hydrophila is bacteria that often infect freshwater fish and causes Motile Aeromonas Septicemia (MAS) disease. One alternative antibacterial that can be used is squid ink (Loligo sp.) which contains antibacterial substances. The purpose of this study was to determine the effect of squid ink (Loligo sp.) on antibody titers and bacterial density in tilapia infected with A. hydrophila.

This study used a Completely Randomized Design (CRD) with the treatment of squid ink powder in feed at a dose of 52.5 ppm (A), 62.5 ppm (B) and 72.5 ppm (C), positive control (K+) using oxytetracycline ( 20 ppm) on feed and negative control (K-) without giving treatment to feed. The main parameters observed were bacterial density in blood of tilapia (O. niloticus) and antibody titer. In addition to the main parameters, supporting parameters observed in this study included survival rate (SR), and measurements of water quality (temperature, DO and pH).

The best dose in giving squid ink (Loligo sp.) powder in feed was 62.5 ppm (treatment B) because it supresing bacterial density in the blood of tilapia (O. niloticus) until 166.33 X 10³ cfu/mL, decreasing antibody titer from 26.67±09.24 % to 13.00±04.62 % and highest survival rate of 90.47%.

Keywords

MAS, O. niloticus, Squid ink, Antibody, Blood

Full Text:

PDF

References

Affandi, R. I., M. Fadjar and A. W. Ekawati. 2019. Active Compounds on Squid (Loligo sp.) Ink Extract Powder as Immunostimulant Candidate to Against Shrimp Disease. Research Journal of Life Science. 6 (3) : 150-161.

Arie, U. 1998. Pembenihan dan Pembesaran Nila Gift. Penebar Swadaya. Jakarta. 128 hlm

Fadjar, M., S. Andajani and K. Zaelani. 2016. Squid (Loligo edulis) ink raw extract as an anti–vibriosis substance in grouper (Epinephelus fuscoguttatus) juvenile culture infected by Vibrio alginolyticus. AACL Bioflux. 9(2) : 422– 428.

Fitrial, Y. dan Khotimah, I. K. 2017. Aktivitas antibakteri dari melanin tinta sotong dan cumi-cumi. Jurnal Pengolahan Hasil Perikanan Indonesia. 20 (2) : 266–274

Hartika, R., Mustahal dan A. N. Putra. 2014. Gambaran Darah Ikan Nila (Oreochromis niloticus) dengan Penambahan Dosis Prebiotik Yang Berbeda Dalam Pakan. Jurnal Perikanan dan Kelautan. 4(4) : 259-267.

Khairuman dan K. Amri. 2011. 2,5 Bulan Panen Ikan Nila. Agromedia Pustaka. Jakarta. 202 p..

KKP. 2014. Ministry of Marine Affairs and Fisheries of the Republic of Indonesia. 214 hlm

Liu, L., Gong, Y.-X., Zhu, B., Liu, G.-L., Wang, G.-X., & Ling, F. (2015). Effect of a new recombinant Aeromonas hydrophila vaccine on the grass carp intestinal microbiota and correlations with immunological responses. Fish Shellfish Immunology, 45, 175–183.

Miranti, S. 2016. Pengendalian infeksi Vibrio harveyi pada udang vaname dengan ekstrak kunyit sambiloto dalam pakan di karamba jaring apung Kepulauan Seribu. Tesis. Institut Pertanian Bogor. 52 hlm.

Mubarak, Z., S. Chismirna dan H. H. Daulay. 2016. Aktivitas antibakteri ekstrak Propolis Alami dari Sarang Lebah TErhadap Pertumbuhan Enterococcus faecalis. J Syiah Kuala Dent Soc. 1(2):175-186.

Mulia, D. S. 2012. Vaksinasi Lele Dumbo. Pustaka pelajar, Yogyakarta. 92 p.

Nazir, M. 2005. Metode Penelitian. Bogor: Ghalia Indonesia Cetakan 6. 88 p

Pelczar, M. J. dan E.C.S. Chan. 2005. Dasar-dasar Mikrobiologi. UI Press, Jakarta. 89 p.

Royan, F., S. Rejeki dan A. H. C. Haditomo. 2014. Pengaruh Salinitas yang Berbeda terhadap Profil Darah Ikan Nila (Oreochromis niloticus). Journal of Aquaculture Management and Technology. 3(2): 109-117

Sadikin, M. 2002, Biokimia Darah.. Widya Medika: Jakarta.p:53.

Sahu, I., B. K. Das, N. Marhual, M. Samanta, B.K. Mishra, and A. E. Eknath. 2011. Toxicity of crude extracellular product of Aeromonas hydrophila on Rohu, Labeo Rohita (Ham.). Indian J. Microbiol. 51 (4): 515-520.

Sari, N. W., I. Lukistyowati dan N. Aryani. 2012. Pengaruh pemberian temulawak (Curcuma xanthorriza Roxb) terhadap kelulushidupan ikan mas (Cyprinus carpio L) setelah di infeksi Aeromonas hydrophila. Jurnal perikanan dan kelautan. 17 (2): 12-19.

Salah, M.A, Aqel, S.A., Arshad, H.R., & Nashwa, M.A.A. (2015). The response of new-season nile tilapia to Aeromonas hydrophila vaccine. International Journal of clinical, 8(3), 4508-4514.

Sucipto, A dan R. E. Prihartono. 2007. Pembesaran Nila Merah Bangkok. Penebar Swadaya. Jakarta. 156 p.

Sugiani, D., Taukhid, U. Purwaningsih, dan A. M. Lusiastuti. 2018. Vaksin Kering Beku Sel Utuh Bakteri Aeromonas hydrophila Untuk Pencegahan Penyakit Motile Aeromonads septicemia pada Ikan Lele, Nila dan Gurami. Jurnal Riset Akuakultur. 13 (2): 59-167.

Taukhid dan U. Purwaningsih. 2011. Penapisan isolat bakteri Streptococcus spp. sebagai sebagai kandidat antigen dalam pembuatan vaksin, serta efikasinya untuk pencegahan penyakit streptococcosis pada ikan nila, Oreochromis niloticus. Jurnal Riset Akuakultur. 6: 103–118.

Wahjuningrum, D., Astrini, R., & Setiawati, M. 2013. Pencegahan Aeromonas hydrophila pada benih ikan lele menggunakan bawang putih dan meniran Prevention of Aeromonas hydrophila on catfish juvenile using garlic and shatterstone herb. Jurnal Akuakultur Indonesia. 12(1): 86–94.

DOI: http://dx.doi.org/10.21534/ai.v21i1.170