Research Update: Development of Plant-based Diets for Florida pompano Trachinotus carolinus

Romi Novriadi, D. Allen Davis


The development of sustainable and economically sound practical diet in the coming years will depend on the reduction of fish meal and the increased inclusion of sustainable protein sources. Over the years, our laboratory has systematically reduced the inclusion levels of fish meal and other animal protein sources by using a range of alternative plant-protein sources. These diets may be further improved through the use of specialized proteins, supplements for limiting amino acids, enzyme and attractants. Good success has been seen with corn protein concentrates, advanced processing products of soybean meal, such as soy protein concentrates and advanced soy product (enzyme treated or fermented soy). Based on the results of our research, the proportion of animal meal can be reduced to 12 % in our current formulations without any detrimental effects on pompano performance and nutrient retention. In addition, the use of fermented soy or the combination of enzyme-treated soy with proper inclusion level of squid hydrolysates as an attractant has proven to improve the functional properties of SBM and lead to better liver and intestine condition of pompano. Considering the sizeable body of knowledge concerning nutrition for Florida pompano, all information taken from this species could be used as the starting point to develop better feed formulations for Silver pompano production in Indonesia.


Alternative protein; Florida pompano; Practical diet Soy-protein; Sustainable

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Amaya, E., Davis, D.A. & Rouse, D.B. (2007) Alternative diets for the Pacific white shrimp Litopenaeus vannamei. Aquaculture, 262, 419-425.

Azarm, H.M. & Lee, S.M. (2014) Effects of partial substitution of dietary fish meal by fermented soybean meal on growth performance, amino acid and biochemical parameters of juvenile black sea bream Acanthopagrus schlegeli. Aquaculture Research, 45, 994-1003.

Barnard, P. (2006) Gustatory and olfactory feeding responces in Japanese koi carp (Cyprinus carpio). Stellenbosch: University of Stellenbosch.

Barnes, M.E., Brown, M.L., Bruce, T., Sindelar, S. & Neiger, R. (2014) Rainbow trout rearing performance, intestinal morphology, and immune response after long-term feeding of high levels of fermented soybean meal. North American Journal of Aquaculture, 76, 333-345.

Barry, K.J., McClure, R.L. & Trushenski, J.T. (2017) Sea Clam-Derived Feeding Stimulants Enhance Acceptability and Intake of Reduced Fish Meal, Soy-Based Sunshine Bass Feeds. North American Journal of Aquaculture, 79, 115-122.

Bi, H., Zhao, H., Lu, F., Zhang, C., Bie, X. & Lu, Z. (2015) Improvement of the Nutritional Quality and Fibrinolytic Enzyme Activity of Soybean Meal by Fermentation of Bacillus subtilis. Journal of Food Processing and Preservation, 39, 1235-1242.

Chen, L., Vadlani, P.V., Madl, R.L. & Gibbons, W. (2016) Degradation of Phytic Acid and Soy Protein in Soy Meal via Co-fermentation of Aspergillus oryzae and Aspergillus ficuum. Journal of the American Oil Chemists' Society, 93, 45-50.

Chi, C.-H. & Cho, S.-J. (2016) Improvement of bioactivity of soybean meal by solid-state fermentation with Bacillus amyloliquefaciens versus Lactobacillus spp. and Saccharomyces cerevisiae. LWT-Food Science and Technology, 68, 619-625.

Cook, R.L., Zhou, Y., Rhodes, M.A. & Davis, D.A. (2016) Evaluation of various cottonseed products on the growth and digestibility performance in Florida pompano Trachinotus carolinus. Aquaculture, 453, 10-18.

Davis, D.A. & Arnold, C. (2000) Replacement of fish meal in practical diets for the Pacific white shrimp, Litopenaeus vannamei. Aquaculture, 185, 291-298.

Gaber, M. (2005) The Effect of Different Levels of Krill Meal Supplementation of Soybean‐based Diets on Feed Intake, Digestibility, and Chemical Composition of Juvenile Nile Tilapia Oreochromis niloticus, L. Journal of the World Aquaculture Society, 36, 346-353.

Gatlin, D.M., Barrows, F.T., Brown, P., Dabrowski, K., Gaylord, T.G., Hardy, R.W., Herman, E., Hu, G., Krogdahl, Å. & Nelson, R. (2007) Expanding the utilization of sustainable plant products in aquafeeds: a review. Aquaculture research, 38, 551-579.

Gothreaux, C.T., Reigh, R.C., Williams, M.B. & Chesney, E.J. (2010) Amino acid availability of soybean meal for Florida pompano. North American Journal of Aquaculture, 72, 309-313.

Hernández, C., Olvera-Novoa, M.A., Smith, D.M., Hardy, R.W. & Gonzalez-Rodriguez, B. (2011) Enhancement of shrimp Litopenaeus vannamei diets based on terrestrial protein sources via the inclusion of tuna by-product protein hydrolysates. Aquaculture, 317, 117-123.

Hong, K.-J., Lee, C.-H. & Kim, S.W. (2004) Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. Journal of medicinal food, 7, 430-435.

Kaushik, S., Cravedi, J., Lalles, J., Sumpter, J., Fauconneau, B. & Laroche, M. (1995) Partial or total replacement of fish meal by soybean protein on growth, protein utilization, potential estrogenic or antigenic effects, cholesterolemia and flesh quality in rainbow trout, Oncorhynchus mykiss. Aquaculture, 133, 257-274.

Kissinger, K.R., García-Ortega, A. & Trushenski, J.T. (2016) Partial fish meal replacement by soy protein concentrate, squid and algal meals in low fish-oil diets containing Schizochytrium limacinum for longfin yellowtail Seriola rivoliana. Aquaculture, 452, 37-44.

Krogdahl, A., Bakke-McKellep, A., Roed, K. & Baeverfjord, G. (2000) Feeding Atlantic salmon Salmo salar L. soybean products: effects on disease resistance (furunculosis), and lysozyme and IgM levels in the intestinal mucosa. Aquaculture Nutrition, 6, 77-84.

Lazo, J.P., Davis, D.A. & Arnold, C.R. (1998) The effects of dietary protein level on growth, feed efficiency and survival of juvenile Florida pompano (Trachinotus carolinus). Aquaculture, 169, 225-232.

Lech, G.P. & Reigh, R.C. (2012) Plant products affect growth and digestive efficiency of cultured Florida pompano (Trachinotus carolinus) fed compounded diets. PLoS One, 7, e34981.

Liu, K. (2012) Soybeans: chemistry, technology, and utilization, Springer.

Mambrini, M., Roem, A.J., Carvedi, J., Lalles, J. & Kaushik, S. (1999) Effects of replacing fish meal with soy protein concentrate and of DL-methionine supplementation in high-energy, extruded diets on the growth and nutrient utilization of rainbow trout, Oncorhynchus mykiss. Journal of animal science, 77, 2990-2999.

Nagel, F., Danwitz, A., Schlachter, M., Kroeckel, S., Wagner, C. & Schulz, C. (2014) Blue mussel meal as feed attractant in rapeseed protein‐based diets for turbot (Psetta maxima L.). Aquaculture Research, 45, 1964-1978.

Ng, A.N., de Jong-Curtain, T.A., Mawdsley, D.J., White, S.J., Shin, J., Appel, B., Dong, P.D.S., Stainier, D.Y. & Heath, J.K. (2005) Formation of the digestive system in zebrafish: III. Intestinal epithelium morphogenesis. Developmental biology, 286, 114-135.

Nordrum, S., Bakke-McKellep, A., Krogdahl, Å. & Buddington, R. (2000) Effects of soybean meal and salinity on intestinal transport of nutrients in Atlantic salmon (Salmosalar L.) and rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 125, 317-335.

Novriadi, R., Spangler, E., Rhodes, M., Hanson, T. & Davis, D.A. (2017a) Effects of various levels of squid hydrolysate and squid meal supplementation with enzyme-treated soy on growth performance, body composition, serum biochemistry and histology of Florida pompano Trachinotus carolinus. Aquaculture, 481, 85-93.

Novriadi, R., Rhodes, M., Powell, M., Hanson, T. & Davis, D.A. (2017b) Effects of soybean meal replacement with fermented soybean meal on growth, serum biochemistry and morphological condition of liver and distal intestine of Florida pompano Trachinotus carolinus. Aquaculture Nutrition. In press DOI:10.1111/anu.12645

Nunes, A.J., Sá, M.V., Andriola-Neto, F.F. & Lemos, D. (2006) Behavioral response to selected feed attractants and stimulants in Pacific white shrimp, Litopenaeus vannamei. Aquaculture, 260, 244-254.

Oehlers, S.H., Flores, M.V., Okuda, K.S., Hall, C.J., Crosier, K.E. & Crosier, P.S. (2011) A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents. Developmental Dynamics, 240, 288-298.

Olli, J., Krogdahl, Å. & Våbenø, A. (1995) Dehulled solvent‐extracted soybean meal as a protein source in diets for Atlantic salmon, Salmo salar L. Aquaculture Research, 26, 167-174.

Papagianni, M., Nokes, S.E. & Filer, K. (1999) Production of phytase by Aspergillus niger in submerged and solid-state fermentation. Process Biochemistry, 35, 397-402.

Paripatananont, T., Boonyaratpalin, M., Pengseng, P. & Chotipuntu, P. (2001) Substitution of soy protein concentrate for fishmeal in diets of tiger shrimp Penaeus monodon. Aquaculture research, 32, 369-374.

Patro, B., Reigh, R. & Williams, M. (2011) Dietary methionine requirement of Florida pompano In Aquaculture America 2011New Orleans, USA.

Phillips, R.D. & Sternberg, M. (1979) Corn protein concentrate: functional and nutritional properties. Journal of Food Science, 44, 1152-1155.

Quintero, H.E., Davis, D.A. & Rhodes, M.A. (2012) Soy Protein Concentrate as an Alternative Ingredient in Florida Pompano (Trachinotus carolinus) Diets. Journal of Applied Aquaculture, 24, 247-261.

Refstie, S., Korsøen, Ø.J., Storebakken, T., Baeverfjord, G., Lein, I. & Roem, A.J. (2000) Differing nutritional responses to dietary soybean meal in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar). Aquaculture, 190, 49-63.

Refstie, S., Olli, J.J. & Standal, H. (2004) Feed intake, growth, and protein utilisation by post-smolt Atlantic salmon (Salmo salar) in response to graded levels of fish protein hydrolysate in the diet. Aquaculture, 239, 331-349.

Rhodes, M., Zhou, Y., Salze, G., Hanson, T. & Davis, D. (2017) Development of plant‐based diets and the evaluation of dietary attractants for juvenile Florida pompano, Trachinotus carolinus L. Aquaculture Nutrition.

Rhodes, M.A., Hanson, T., Alavi, S. & Davis, A.D. (2013) High-soy, fishmeal-free diets support Florida pompano growth In Global Aquaculture Advocate, Vol. September/October, pp. 96-99St Louis, Missouri.

Rhodes, M.A., Zhou, Y. & Davis, D.A. (2015) Use of Dried Fermented Biomass as a Fish Meal Replacement in Practical Diets of Florida Pompano,Trachinotus carolinus. Journal of Applied Aquaculture, 27, 29-39.

Rhodes, M.A., Zhou, Y., Salze, G.P., Hanson, T.R. & Davis, A.D. (2017b) Development of plant- based diets and the evaluation of dietary attractants for juvenile Florida pompano, Trachinotus carolinus L. Aquaculture Nutrition.

Riche, M. (2011) Estimated quantitative amino acid requirements for Florida pompano reared in low-salinity environments In Aquaculture America 2011. World Aquaculture Society, New Orleans, LA.

Riche, M. (2015) Nitrogen utilization from diets with refined and blended poultry by-products as partial fish meal replacements in diets for low-salinity cultured Florida pompano, Trachinotus carolinus. Aquaculture, 435, 458-466.

Riche, M. & Williams, T. (2010) Apparent digestible protein, energy and amino acid availability of three plant proteins in Florida pompano, Trachinotus carolinus L. in seawater and low‐salinity water. Aquaculture Nutrition, 16, 223-230.

Riche, M. & Williams, T.N. (2011) Fish meal replacement with solvent-extracted soybean meal or soy protein isolate in a practical diet formulation for Florida pompano (Trachinotus carolinus, L.) reared in low salinity. Aquaculture Nutrition, 17, 368-379.

Roe, C. (2016) Improving High Soy Feed Formulations for Florida Pompano Trachinotus carolinus through Phytase Supplementation. In Fisheries, Aquaculture and Aquatic Sciences, Vol. Mastrs fo Science, pp. 60. Auburn University, Auburn.

Rombenso, A.N., Trushenski, J.T. & Schwarz, M.H. (2016) Fish oil replacement in feeds for juvenile Florida Pompano: Composition of alternative lipid influences degree of tissue fatty acid profile distortion. Aquaculture, 458, 177-186.

Rombenso, A.N., Trushenski, J.T. & Schwarz, M.H. (2017) Beef tallow is suitable as a primary lipid source in juvenile Florida pompano feeds. Aquaculture Nutrition

Rossi Jr, W. & Davis, D.A. (2012) Replacement of fishmeal with poultry by-product meal in the diet of Florida pompano Trachinotus carolinus L. Aquaculture, 338–341, 160-166.

Rossi, W. & Davis, D.A. (2012) Replacement of fishmeal with poultry by-product meal in the diet of Florida pompano Trachinotus carolinus L. Aquaculture, 338, 160-166.

Rossi, W. & Davis, D.A. (2014) Meat and Bone Meal as an Alternative for Fish Meal in Soybean Meal-Based Diets for Florida Pompano, Trachinotus carolinus L. Journal of the World Aquaculture Society, 45, 613-624.

Rumsey, G.L., Siwicki, A.K., Anderson, D.P. & Bowser, P.R. (1994) Effect of soybean protein on serological response, non-specific defense mechanisms, growth, and protein utilization in rainbow trout. Veterinary Immunology and Immunopathology, 41, 323-339.

Salze, G., Rhodes, M. & Davis, D.A. (2014) Estimation of the quantitative requirement for dietary taurine in Florida pompano, Trachinotus carolinus, Juveniles In Aquaculture America 2014Seattle, USA.

Salze, G.P. & Davis, D.A. (2015) Taurine: a critical nutrient for future fish feeds. Aquaculture, 437, 215-229.

Salze, G.P., Spangler, E., Cobine, P.A., Rhodes, M. & Davis, D.A. (2016) Investigation of biomarkers of early taurine deficiency in Florida pompano Trachinotus carolinus. Aquaculture, 451, 254-265.

Sookying, D. (2010) Development and application of soybean based diets for Pacific white shrimp Litopenaeus vannamei, Auburn University.

Sookying, D., Davis, D. & Soller Dias Da Silva, F. (2013) A review of the development and application of soybean‐based diets for Pacific white shrimp Litopenaeus vannamei. Aquaculture Nutrition, 19, 441-448.

Tatum, W.M. (1972) Comparative Growth, Mortality and Efficiency of Pompano (Trachinotus Carolinus) Receiving a Diet of Ground Industrial fish with those receiving A Diet of trout chow1 In Proceedings of the annual workshop‐World Mariculture Society, Vol. 3, pp. 65-74. Wiley Online Library.

Tibaldi, E., Hakim, Y., Uni, Z., Tulli, F., de Francesco, M., Luzzana, U. & Harpaz, S. (2006) Effects of the partial substitution of dietary fish meal by differently processed soybean meals on growth performance, nutrient digestibility and activity of intestinal brush border enzymes in the European sea bass (Dicentrarchus labrax). Aquaculture, 261, 182-193.

Urán, P., Schrama, J., Rombout, J., Taverne‐Thiele, J., Obach, A., Koppe, W. & Verreth, J. (2009) Time‐related changes of the intestinal morphology of Atlantic salmon, Salmo salar L., at two different soybean meal inclusion levels. Journal of fish diseases, 32, 733-744.

Wang, L., Zhou, H., He, R., Xu, W., Mai, K. & He, G. (2016) Effects of soybean meal fermentation by Lactobacillus plantarum P8 on growth, immune responses, and intestinal morphology in juvenile turbot (Scophthalmus maximus L.). Aquaculture, 464, 87-94.

Weirich, C.R. & Riley, K.L. (2007) Volitional Spawning of Florida Pompano,Trachinotus carolinus, Induced via Administration of Gonadotropin Releasing Hormone Analogue (GnRHa). Journal of Applied Aquaculture, 19, 47-60.

Williams, S., Lovell, R.T. & Hawke, J.P. (1985) Value of menhaden oil in diets of Florida pompano. Progressive Fish Culturist, 47, 159-165.

Yamamoto, T., Iwashita, Y., Matsunari, H., Sugita, T., Furuita, H., Akimoto, A., Okamatsu, K. & Suzuki, N. (2010) Influence of fermentation conditions for soybean meal in a non-fish meal diet on the growth performance and physiological condition of rainbow trout Oncorhynchus mykiss. Aquaculture, 309, 173-180.

Zhuo, L.C., Liu, K. & Lin, Y.H. (2014) Apparent digestibility of soybean meal and Lactobacillus spp. fermented soybean meal in diets of grouper, Epinephelus coioides. Aquaculture Research.



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